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Image Search Results
Journal: Journal of pharmacological sciences
Article Title: The Class I PI3K inhibitor S14161 induces autophagy in malignant blood cells by modulating the Beclin 1/Vps34 complex.
doi: 10.1016/j.jphs.2017.07.001
Figure Lengend Snippet: Fig. 2. S14161 induces LC3-II expression in myeloma and leukemia cells. AeB, S14161 induced LC3-II expression in a concentration-dependent manner. RPMI-8226 cells were treated with S14161 (0, 10, 20 mM) or Idelalisib (0, 100, 200 mM), or Wortmannin (0, 1, 2 mM) for 24 h, LC3 expression were detected by Western blot. C, S14161 induced LC3-II expression in various cancer cells. MM cells (OPM2, RPMI-8226, KMS11) and leukemia cells (K562) were treated with 20 mM S14161 or DMSO for 10 h. After incubation, cells were processed for analysis of LC3 and GAPDH by Western blot. D, S14161 induced LC3-II expression in a time-dependent manner. KMS11 and K562 cells were treated with 10 mM S14161 at 0, 12, 24 or 36 h, and then LC3 was detected by Western blot. E, S14161 induced LC3-II expression analyzed by a confocal microscope. K562 and OPM2 cells were starved or treated with S14161 (20 mM) alone or combination with 3-MA (0.2 mM) or BafA1 (20 nM) for 10 h. Then, cells were fixed and stained with antibodies against LC3 (red color) and DAPI (blue color) before being applied to analyze on a confocal microscope. Autophagosome formation was defined by the accumulation of LC3, arrows indicate autophagosomes. The expression ratio of LC3II over GAPDH was analyzed by densitometry.
Article Snippet: Horseradish peroxidase-conjugate secondary antibodies,
Techniques: Expressing, Concentration Assay, Western Blot, Incubation, Microscopy, Staining